I need some help regarding vaccine potency testing and dose calculation for live and killed viral?

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Salameh Barhoom

About the question: This is open one include all vaccinology science ,so please ask problem you face during your work to facilitate it according to my ability.

Best regards

Muhammad Usman Qamar

Salameh Barhoom I am working on LSD live attenuated vaccine and I want to check its potency and determine the dose. I don't know how to check its potency by using TCID50 as well as how can determine its dose?

Thank you

LSD vaccine available is sheeppox vaccine live attenuated vaccine administrted Intradermanelly or s/c dose used in cattle 8 doses of sheep for cattle.Scientfically dose calculated routinelly by Reed and Munech or karby method TCID50 ,until know no applied scientfic dose for LSD disease.