There is a protocol in the site protocolsonline.com for Weil's myelin stain. I have no experience with this particular staining method
Dear Anosh Saleem , it seems to be known why you'll use a staining method said to be a bit outdated (implemented & published 1928)...You'll have for sure an idea why...would you share these thoughts with us...??
By the way, from (quote-s, 'anonymous' blog Dec 4, 2018, ) :
https://brainstuff.org/blog/weil-stain-brain :
>>A Weil stain is an experimental anatomical technique that is used on thinly sliced sections of nervous tissue, including the brain or spinal cord. The Weil stain was first published in 1928, so it has been largely replaced by more advanced techniques that allow for higher resolution images to be captured. First, the tissue samples must be fixed with formalin, and then embedded in paraffin. Thin slices of tissue between 20 and 50 microns will allow the best staining.
The chemistry of the Weil stain relies on the chrome solution that the brain sections are immersed in. The reaction with the myelin sheaths creates chromium dioxide, which then reacts with a hematoxylin step. When the stain is completed, myelin will appear black which should stand out boldly against the clear or yellow background tissue.
Tagged: Weil stain, Methods, Laboratory techniques, Laboratory methods, Myelin, Staining, Axon, White matter
Jose Silva's hint to >protocolsonline.com< =
https://www.protocolsonline.com/histology/dyes-and-stains/neurohistology/weils-myelin-stain/
seems to be really a good source and protocol...
Here to add only (for historical reason):
(QUOTE/cited from:
Terminology of Staining. Online Glossary.
by jkiernan | Feb 11, 2019 | News About Dyes and Stains |
https://biologicalstaincommission.org/bscglossary.html
>>Weil. Arthur Weil (1887‑1969) was a German physician, biochemist, endocrinologist and neuropathologist who moved to the USA in 1921 and was a professor of pathology at Northwestern University, Chicago, 1928‑1944. While there, he devised a rapid staining method for myelin sheaths with iron hematoxylin as well as silver staining methods for neuroglial cells. From 1944 until his retirement in 1963 Weil conducted research in a wide variety of fields at Mount Sinai Hospital, New York, and also edited the Journal of Neuropathology & Experimental Neurology for many years. Weil’s stain for myelin. A staining technique similar to Weigert’s hematoxylin method. Sections are over-stained with a freshly mixed iron hematoxylin solution and differentiated first in a solution of ferric ammonium sulfate and then in an alkaline solution of potassium ferricyanide. Myelin sheaths and erythrocytes are black. This technique, introduced by Weil in 1928, is simpler and less time consuming than the traditional myelin stains devised by Weigert (1885) and Pal (1887). The phrase “Weigert-Pal stain” is often applied to any method that uses a metal coordination complex of hematein to stain myelin black.