I may have a contaminant agent in my HEPG2 cells, but don't know what is it?

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How do you collect the celiac ganglion from mice?

Does anyone know how to / anatomical landmarks to collect the celiac ganglion?

06 December 2023 8,709 0 View

Can you tell me a protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

Hello everyone, Can you tell me a standardized protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

14 November 2023 1,546 1 View

Freeman Tukey test: is there an online tool?

Hi, I have data in form of counts (number) and I wanted to run a statistical test to compare the counts between groups to see if there is statistically significant difference between groups. I...

24 October 2023 1,545 3 View

How can I prevent exosomes from clumping together in PBS for NTA after isolation?

I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...

13 August 2023 4,773 0 View

Has anyone had problems with the PyroMark Q48 cartridges?

Hi everyone, a few months ago we bought the PyroMark Q48 to replace the old Q96, but we used it very little due to continuous malfunctions of the cartridge with enzyme and substrate. We've already...

23 July 2023 8,854 0 View

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices?

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices? Can someone provide a study on the dose-response relationship of glutamate in brain slice preparations?

11 July 2023 9,547 1 View

At which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

at which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

13 June 2023 7,880 2 View

What to do when my ANOVA analysis has a medium effect size but a non-significant p-value?

Hi everyone. I am running an ANOVA analysis, and there is an interaction with a medium partial eta squared value (.06). Still, the p-value is insignificant ( .11). I have calculated the sample...

06 June 2023 3,434 6 View

Is it possible to quantify 16S regions in a sample of WGS data?

Hi, I would like to know if there is any software readily available which allows the identification and quantification for 16S regions in WGS data. Thanks n advance for any advice/help :)

15 May 2023 1,177 0 View

How do you estimate parts of the biofilm being sediments or dead organic material of biomass collected from river stones?

We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...

27 March 2023 2,413 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View