I have to isolate genomic dna from dried leaf beetle samples and I'm planning to do that using the Nucleospon Insect DNA kit. What should I do ?

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What are the procedures for environment friendly and effective methods to prepare silicon QDs, Si nanotubes and silicene (2D materials)?

There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...

10 January 2021 3,418 1 View

What are the methods to eliminate or minimize total dissolve solids in industrial effluent ?

technology relevant methods or processes

02 January 2021 6,632 2 View

Does the adsorbed water or gases affect the CHNS analysis of high surface area porous carbon ?

Adsorbed water and gases can affect the weight of the porous carbon. Some times 6-7% weight loss can occur during the degassing of the sample. Will this weight loss affect the calculation of C,...

08 November 2020 5,399 3 View

What is RNA filtration technique?

Sir, How I Can filter the only RNA as well as DNA separately from the whole genome after lysing using a membrane, and which membrane filter is suitable for the same.

14 September 2020 601 1 View

How to solve this error in a Heat Exchange Reformer model in Aspen Custom Modeler?

I have written code for a for a Heat Exchange Reformer in Aspen Custom Modeler, but it cannot run. The program describes a shell and tube heat exchanger with steam methane reforming reactions...

07 September 2020 2,024 9 View

Why is that only vaccine is available for Hepatitis B and not a drug that can eradicate it?

Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...

22 June 2020 5,661 3 View

What is the physical significance of order (q) when calculating the Information entropy?

In information theory, the entropy of a variable is the amount of information contained in the variable. One way to understand the concept of the amount of information is to tie it to how...

01 May 2020 6,703 6 View

What are the simulation tools for IoT ?

For academic purpose, free and open source simulation tool for internet of things

09 April 2020 7,137 11 View

How to identify the concentration and weight used for magnetic composite preparation ?

I have got a publication in which the authors prepared BaFe12O19/CoFe2O4. But the amount / concentration used for preparing the metal nitrate solutions were not mentioned in the publication...

15 January 2020 3,563 2 View

How to find the human chromosome DNA sequence ? Where I can get exact data ?

I already found the human chromosome DNA sequence from the ensemble browser .But the problem is in that sequence it shows some N terms are coming top , bottom and in the middle .Without N terms...

12 December 2019 7,218 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View