I have to isolate genomic dna from dried leaf beetle samples and I'm planning to do that using the Nucleospon Insect DNA kit. What should I do ?

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Why RNA pellets of testis samples become gel during RNA isolation ?

I have been trying to isolate RNA from magur fish testis, at the end of RNA isolation, RNA pellets becoming gel when DEPC water is added, what could be the possible reason for that? What is the...

05 May 2024 5,278 1 View

Why got population shift in flow cytometer?

Hello connection While performing potency test by using BD FACS instrument some time we few population inside the gate few outside of gate and some time I got population outside of the gate I am...

17 March 2024 3,511 4 View

Why are there large differences between the expected deformation gradient (DG) and the DG printed by VUMAT in 2D simple shear example?

As demonstrated in the affixed file, there are large differences between the expected deformation gradient (DG) and the DG printed by VUMAT in 2D simple shear example. The differences are...

21 February 2024 4,851 0 View

Whether the XRD and FT-IR of the material before and after the photocatalytic reaction can be compared to know the change in structure of the mat. ?

Kindly provide the details

30 August 2023 4,884 1 View

How to calculate standard free energy change for the following reaction using Born-Haber cycle using DFT?

I am referring Theoretical studies on electrochemistry of p-aminophenol paper for calculating the theoretical redox potential using the described in this paper. I reproduce the DFT calculations...

18 August 2023 3,573 1 View

How to calculate the free energy change of solvation for system A + 2H(+) + 2 e(-) --> AH2 to calculate the theoretical redox potential?

I encountered the calculations in the research article titled "Theoretical studies on electrochemistry of p-aminophenol ." However, I'm having difficulty comprehending the procedure used to...

13 August 2023 1,639 1 View

Inquiry regarding the thermal kinetics observed in a Differential Scanning Calorimetry (DSC) analysis?

I have a query is that after crystallization peak point some of other noise type peaks occur in DSC scan for (Se78-xTe20Sn2Znx) x=2 and x=6 ,this peak shown the material property or due to...

05 August 2023 8,945 1 View

Gaussian 16, maximum force is not converged, all other parameters converged. How to solve this issue?

I am trying to optimize the geometry of a reactant complex obtained from the IRC. The geometry of the product complex is getting optimized, but in the reactant complex, the maximum force is not...

23 May 2023 8,098 3 View

Why I am not able to isolate the plasmid after get colony PCR postive?

Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...

16 May 2023 884 3 View

Why my cloning are not working ?

Hello everyone, I have one doubt regarding cloning ? I am using pET30a (+) vector for cloning (5422bp) and Insert size 804bp. I tried all the ligation reaction ratio lilke 1:3, 1:5, 1;7 (100ng...

26 March 2023 7,023 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View