I have studied MB dye decolorization study using bacteria and bacteria with ZnO NP in same solution.what would be reason for enhanced reaction?

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Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

While doing docking using Autodock1.5.7, I am getting a warning that there are non integral charges on my ligand. How to remove it?

I used Autodock for docking, due to the non integral charges on my ligand I am not able to dock with the protein. How to dock the file?

08 June 2024 5,593 2 View

Total phenol content estimation?

The addition of Na2CO3 in TPC estimation creates an alkaline medium for phenols to get unstable, however whenever I add 2 ml of 20% of Na2CO3 to 1 ml of gallic acid extract and 0.5 ml of FC...

14 May 2024 5,478 2 View

Can someone help me out for understanding compartments and non compartment model ???

I am facing some what difficulty in understand different types of compartment and non compartment model. So can someone help me out in this ??

13 May 2024 931 0 View

Validation of the formula for beta-carotene estimation?

For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm...

16 April 2024 3,739 1 View

What is apparent density?

There is a define calculation method of apparent specific gravity. But do we have a method to calculate apparent density? is bulk density and apparent density same? or different ?

13 March 2024 3,513 0 View

Protocol for purification of his tagged alpha synuclein?

Hi, I am trying to purify alpha synuclein protein from E.coli BL21(DE3) cells. The plasmid is pET21a alpha-synuclein. Can anyone help me with the protocol. Thank you

15 January 2024 8,876 2 View

I want to culture and expand MSCs in serum free condition to isolate small EVs. What is the alternative to FBS If I have DMEM KO as basal media??

I'd prefer not buying any separate commercial media because of the cost. Please suggest what growth factors or vitamins can I add to DMEM KO other than glutamax, penstrep and bFGF for the MSCs to...

08 January 2024 6,443 3 View

What are the values of electronic properties of Niobium Nitride (NbN)?

Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...

02 January 2024 3,962 1 View

Investigation on structural, hysteresis, Mössbauer properties and electrical parameters in X-type Ba2Co2ErxFe28-xO46 hexaferrites?

Conducted an extensive study on hexaferrites, substituting Erbium into X-type Ba2Co2ErxFe28-xO46 through a precise heat treatment process. XRD analysis highlighted phase variations, showcasing...

16 November 2023 2,147 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

How can magnetic Biochar remove dye pollutants, is this technology beneficial?

The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

07 July 2024 8,118 1 View

Sybergreen qpcr troublshoulting?

500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...

06 July 2024 5,478 4 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View

Performance Standards for Antimicrobial Disk Susceptibility Tests, 14th Edition ?

Performance Standards for Antimicrobial Disk Susceptibility Tests, 14th Edition , please shre this file if anyone have.

29 June 2024 6,929 1 View

What are the key considerations for selecting the temperature in the thermal regeneration process of adsorbents?

I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....

28 June 2024 1,867 3 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

Why cells are visibly pink after DHE staining?

Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...

19 June 2024 7,339 0 View