There are somewhat different recipes for CTAB DNA extraction buffers. One recipe is as follows:
- 2% (w/v) cetyltrimethylammonium bromide (CTAB)
- 100 mM Tris-Cl (pH 8.0)
- 20 mM EDTA (pH 8.0)
- 1.4 M NaCl
- 1% PVP 40,000 (polyvinyl pyrrolidone)
- 0.2% β-mercaptoethanol
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The purpose of the ingredients is as follows:
- CTAB is a cationic surfactant/detergent. CTAB was established some time ago as the best detergent to use during the extraction/isolation of highly polymerized DNA from plant material. This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins.
- Tris-Cl is the buffer system to prevent changes in the pH of the solution.
- EDTA is a chelating agent and has great affinity with divalent metal ions. A Mg-ion is present in DNase as a cofactor and it is responsible for DNase action which degrades the DNA. EDTA snatches away the Mg-ions and nullifies the action of any DNase.
- The role of NaCl: High concentrations of inert salt are used to remove polysaccharides.
- Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds.
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Except for SDS, I do not know which ingredients are in your shampoo, but I am pretty sure that they are quite different from the CTAB buffer. Alone the mentioned SDS makes a big difference: sodium dodecyl sulfate (SDS) is an anionic detergent, i.e. it is negatively charged. In contrast, CTAB is a cationic detergent (positively charged).
I know that there are some simple protocols for isolation of DNA using shampoo (+ salt) to isolate DNA. However, these simple protocols are mainly used for educational purposes in schools to show the principle of DNA extraction. It is not the method of choice if pure DNA with high quality is required.
In summary it can be said that if you want to obtain pure plant DNA, using your shampoo is inappropriate.
There are somewhat different recipes for CTAB DNA extraction buffers. One recipe is as follows:
- 2% (w/v) cetyltrimethylammonium bromide (CTAB)
- 100 mM Tris-Cl (pH 8.0)
- 20 mM EDTA (pH 8.0)
- 1.4 M NaCl
- 1% PVP 40,000 (polyvinyl pyrrolidone)
- 0.2% β-mercaptoethanol
------------------
The purpose of the ingredients is as follows:
- CTAB is a cationic surfactant/detergent. CTAB was established some time ago as the best detergent to use during the extraction/isolation of highly polymerized DNA from plant material. This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins.
- Tris-Cl is the buffer system to prevent changes in the pH of the solution.
- EDTA is a chelating agent and has great affinity with divalent metal ions. A Mg-ion is present in DNase as a cofactor and it is responsible for DNase action which degrades the DNA. EDTA snatches away the Mg-ions and nullifies the action of any DNase.
- The role of NaCl: High concentrations of inert salt are used to remove polysaccharides.
- Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds.
------------------
Except for SDS, I do not know which ingredients are in your shampoo, but I am pretty sure that they are quite different from the CTAB buffer. Alone the mentioned SDS makes a big difference: sodium dodecyl sulfate (SDS) is an anionic detergent, i.e. it is negatively charged. In contrast, CTAB is a cationic detergent (positively charged).
I know that there are some simple protocols for isolation of DNA using shampoo (+ salt) to isolate DNA. However, these simple protocols are mainly used for educational purposes in schools to show the principle of DNA extraction. It is not the method of choice if pure DNA with high quality is required.
In summary it can be said that if you want to obtain pure plant DNA, using your shampoo is inappropriate.
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