Does anyone have experience with Replica plating technique?

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What is the different between impact factor and citescore?

The most journals has impact factor, but some had impact factor and citescore

07 August 2018 1,856 2 View

Why are parsley extract induced alkaline urine secretion in mice?

We study the parsley extract effect in kidney stone, we are observed, the Ph of urine of treated animals was alkaline compared to control

09 October 2017 3,567 0 View

The ratio of DNA /protein is considered an indicator of DNA damage?

We study the effect of acrylamide on bean root tips, then extract the DNA and measure its concentration by specterophotomer at 260 and 280 nm

08 September 2017 5,897 2 View

What is the appropriate media for streptomyces clavuligerus to produce clavulanic acid?

I would like to isolate clavunic acid as beta-lactam antibiotic inhibitor in some g+ve bacteria experiment.

08 September 2017 2,456 6 View

Why neem extract decrease total RBC, when increase the tolal WBC in animals?

we observed that the neem extract decreased total RBC when increased WBC in different neem extract concentration exposed fish.

08 September 2016 5,346 2 View

I have not CTAB buffer. Can I use shampoo instead of CTAB buffer to get pure DNA extraction of plant tissues?

The shampoo contain SDS detergent

08 September 2015 1,450 2 View

Please, help me to know FRAP method of plant extraction?

I am working on some plant extraction antioxidant activity

07 August 2015 4,880 0 View

Do anyone has protocol of simple method for spectrophotometric estimation of phytochemical component ?

I work to determination of plant extract phytochamicals

02 March 2015 4,411 7 View

Does anyone have experience with the preparation of human lymphocyte karyotype?

I would like to study the effect of some plant extract on lymphocyte karyotyping

11 December 2014 9,521 2 View

Can anyone give me phytochemical screening methods of plant seeds

I work on effect of plant seeds extraction on antibiotic resistance bacteria

06 July 2014 5,685 9 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View