I have data for miRNA microarray (agilent platform) using genespring software.How do we manually calculate the normalization factor ?

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Yi-An Chen

Genespring already provides some normalization methods (percentile, quantile...) when you import the raw-data. I guess you are going to apply a normalization method which is not provided by your version of Genespring.

In my case, I performed the normalization with R (Python or other software), then imported the normalized data into Genespring.

Prachi Kochhar

Can you please send a example data sheet for manually calculate normalization factor/ intraarray 90th percentile shift normalization value without using the gene spring software?