Prachi Kochhar

6 Questions 7 Answers 0 Followers

Questions related from Prachi Kochhar

How to use normfinder using Ct values obtained from qPCR?

I have raw Ct values for the 3 housekeeping genes. I want to check which one is the most stable gene using Normfinder. Normfinder software cannot take directly raw Ct values for finding the...

09 December 2019 8,534 0 View

Regarding the cell culture media preparation?

1.After adding antibiotic to the 500ml of DMEM media, can I aliquot the media in 50 ml falcon tubes and freeze at -20°C OR I need to keep at 4°C freezer after adding the antibiotic to the DMEM...

22 February 2019 7,667 3 View

I have data for miRNA microarray (agilent platform) using genespring software.How do we manually calculate the normalization factor ?

Is anyone expert in gene spring software has tried to calculate the normalization factor manually for miRNA microarray data?

05 January 2018 3,528 2 View

Could you please suggest me the housekeeping genes and target gene which are specific to human saliva ?

I have extracted RNA and DNA from human buccal cells.I want to know if it is contaminated with micro flora. Could you please suggest me the housekeeping genes and target gene which are specific to...

07 July 2016 5,177 3 View

Can anyone suggest me the extraction protocols from saliva.I have used trizol method but the yield was low.I don't want to use the kit method?

18 June 2016 9,341 5 View

Hi, Can anyone suggest me the extraction protocols from saliva .I don't want to use the kit method since it is costly.Please suggest me?

Hi, Can anyone suggest me the extraction protocols from saliva which will give high yield.I have tried trizol method but the yield was low.I don't want to use the kit method since it is...

18 June 2016 9,150 2 View