How to interpret Generalised Linear Model output from SPSS?

I am trying to run an ordinal logistic regression using Generalised Linear Model from SPSS. There variables are A,B,C,D, and a moderation variable A*C. Variables A and D are continuous variables...

02 December 2020 5,159 3 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Case-based review vs Case report?

Hello, I have recently stumbled upon a couple of articles consisting of 'case-based review' as the title of their publication. May I ask how different is a case-based review compared to a case...

29 November 2020 8,732 2 View

How to deal with a Solver Pivot Error in Ansys (piezoeletric sensors simulation)?

I am trying to simulate the behaviour of a Piezoelectric sensor, positioned behind a plate subjected to ad impact and consequential oscillations. I have set a transient analysis and associate the...

17 November 2020 3,377 3 View

It is possible to model piezo electric behaviour with Coupled Field Transient tool?

I am trying to model a piezoelectric sensor attached to an aluminum thin plate. I know that it is possible to model a PZT using Mechanical APDL, selecting the proper elements, such as PLANE13,...

13 October 2020 7,915 11 View

How to save displacement output derived in Ansys/Autodyn and use it as input in other environment?

I conducted an analysis in autodyn saving at gauges points the displacements along z. Now, I would need to use this output data as input for analysis in the static structural environment. Since I...

15 September 2020 5,201 3 View

Is Cataglyphis genus present in the Arabian Peninsula?

I found contradictory information about this genus and I would like to understand its correct geographical range.

13 December 2019 8,348 8 View

Is there a rule for how many parameters I can fit to a model, depending on the number of data points I use for the fitting?

I have a model that needs calibration, but I am afraid that if I calibrate using too many model parameters, I will overfit to my data, or the calibration will not be well-done. Can anyone suggest...

27 September 2019 9,480 4 View

Does anyone know a program to create a questionnaire and analyze its statistics?

I'm currently working at a questionnaire for a survey in the field of language education (Italian as a second language education in the Venetian region). I will put the questionnaire online and...

03 September 2019 6,240 7 View

What is the effect of aggressive competition on the industry innovation?

Looking at the competition in the product and service industry, I am curious to know the role of innovation in creating a competitive edge in different industries.

12 August 2019 4,734 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View