I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Why does the ghost atoms gives error in NICS calculation with wb97xd in gaussian09 ?

In a trial, I am trying to calculate NICS (0 and 1) values for benzene ring using gaussian09. I have placed the ghost atoms at the center, 1A, and 2A above the rings. On using the method/basis...

19 January 2021 3,188 3 View

Rejected manuscript for journal publish after 1st revision, what needs to be done next?

Hello, My manuscript has been rejected by the editor and reviewers even after the 1st revision. Though I tend to disagree some of the points raised, assume I can't request for another revision...

28 December 2020 6,062 3 View

Acetylene reduction assay procedure?

Do anyone have the detailed step wise procedure(practical) to do the acetylene reduction assay? Or if anyone can let me know in which institute can i analyse my samples in India?

28 December 2020 6,717 2 View

What is the actual basis on which amount of Perovskites materials are to be taken?

In some discussion people took 112 mg of MAI and 461 mg of PbI2 in 1 mL DMF and some took 461mg of PbI2 too but 159mg of MAI there are many other authors took different amount of these materials...

17 September 2020 9,998 3 View

Forums to get online survey responses?

For my journal publish, I have been asked to increase the sample size by 200. I have been individually contacting more than 1000 Linkedin contacts and posted in all professional groups. But have...

04 September 2020 2,564 5 View

Derivation of Rice and Tracey equation???

12 July 2020 6,555 1 View

Can anyone suggest me where to get a low cost 2D Heterostructure Transfer System?

Except the 2D transfer system sold by HQ Graphene

09 July 2020 3,276 4 View

Why do mangroves intake oxygen through aerating roots like pneumatophores?

Many mangroves like Avicennia and Sonerratia species have developed pneumatophores as an adaptive strategy to survive in hypoxic environment. These pneumatophores receive oxygen from the...

16 April 2020 2,203 3 View

What is a popular book for understanding laser fundamentals and evolution and types of lasers?

I looking for books, channels, videos and PDFs regarding laser and particle interaction and evolution of laser power and laser types and how laser makes the physical changes on the ceramic...

05 April 2020 3,396 25 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View