I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?
Get a medium for bacterial growth into a bacterial-growth tube (plastic or glass), take the stab out, touch it with sterile pipette tip and throw the tip into glass or plastic tube. Then culture overnight or whatever time needed. You will get lots of bacteria in the tube.
Or you can get an agar plate and touch it with the stab all over the place, then you will get your bacteria on the agar plate.
Agree with Yulia. Use selective/ differential medium as per recommendation for your bacterial strain. Use pure culture isolation method.
The easiest method, with good success, is to use a needle (like a loop, but only straight, available in plastic single use or metal reusable) and stab it into the same line of previous stab/growth. When the needle has touched some area of growth, the needle can then be place into a general or enriched broth medium and incubated. Once growth has begun, it is then good to use a loop to sample the broth culture and then swab (for isolation is best) onto an agar plate surface. Using a selective or differential medium may provide some assurance the original species/strain is the one growing.
Yulia Panina Robert John Wolff Yogesh Kumar Negi Thanks to all for valuable feedback.
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