Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run??

More Ramiz Raza's questions See All

Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria?

Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?

31 December 2019 3,589 1 View

Can anybody say which calcium binding agent (which either fluoroscence or give colours)are use to measure calcium level outside the cell or in media ?

Can anybody say which calcium binding agent (which either fluoroscence or give colours) are use to measure calcium level outside the cell or in extracellular media ?

07 August 2019 5,929 2 View

If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?

07 August 2019 7,515 4 View

I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods??

I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods? please give suggestions.

07 August 2019 3,710 12 View

How to track multiple particles or bacteria in ImageJ?

How to track multiple particles or bacteria in ImageJ? I am using multitracker in imageJ but unable to track many particles and bacteria. please help and give valuable suggestions.

06 July 2019 3,405 9 View

How do make a glycerol stock of bacteria for future use?

How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.

01 February 2019 3,626 3 View

I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?

01 February 2019 3,040 4 View

Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it?

Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity...

01 February 2019 7,864 5 View

L-Form of Bacteria?. How can we make E.coli L-form i mean what is the protocol to make it L-form?. How can i maintain this L-form to longer time?

What is L-Form of Bacteria. How can we make E.coli L-form i mean what is the protocol to make it L-form. How can i maintain this L-form to longer time. If not , from where can we order simple...

08 September 2018 7,854 0 View

If bacterial stock is contaminated with other bacteria, How can be sure that whether it is contaminated or not?

Everytime i found different OD data which was fluctuating and sometimes continue increase of OD .Though growth curve and colony under microscope dont tell wheather it is contaminated or not.If...

08 September 2018 461 15 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Bhairavi Vajaria

http://www.ele.uri.edu/courses/bme362/handouts/PDMS_paper.pdf

Article Solid phase DNA extraction on PDMS and direct amplification

https://www.semanticscholar.org/paper/Immobilization-of-DNA-onto-poly(dimethylsiloxane)-a-Liu-Perdue/954ba9710ea582341e3af38962959c2d40e6a0da

Vincent Paul Schmitz

PDMS has been used with ~35-50nt oligonucleotides with no issues, certain harsh solvents may cause swelling but you won't have a problem with standard buffers. Wattman makes several different sizes (https://www.gelifesciences.com/en/us/solutions/lab-filtration/knowledge-center/a-guide-to-whatman-filter-paper-grades), alternatively if you look at it under a microscope with a reference you can directly measure it, they're usually on the order of um.