Try using Kalman filter plugin

https://imagej.nih.gov/ij/plugins/kalman.html

@Aparna Sathya Murthy Can you say how do perform Kalman Filter plugin?. I have tried once but there is no option for multi tracking

Have you tried this : https://imagej.nih.gov/ij/plugins/multitracker.html

Aparna Sathya Murthy yes I have tried but unable to track so if u know something please share

I used ImageJ long ago !!! it is very effective tool !!

I use Matlab : https://www.mathworks.com/help/vision/examples/motion-based-multiple-object-tracking.html

This link is motion detection and tracking . You can try

You can try neuron j also

Follow

Follow

Not my soecilization