Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria?

Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?

31 December 2019 3,589 1 View

Can anybody say which calcium binding agent (which either fluoroscence or give colours)are use to measure calcium level outside the cell or in media ?

Can anybody say which calcium binding agent (which either fluoroscence or give colours) are use to measure calcium level outside the cell or in extracellular media ?

07 August 2019 5,929 2 View

If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?

If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?

07 August 2019 7,515 4 View

I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods??

I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods? please give suggestions.

07 August 2019 3,710 12 View

How to track multiple particles or bacteria in ImageJ?

How to track multiple particles or bacteria in ImageJ? I am using multitracker in imageJ but unable to track many particles and bacteria. please help and give valuable suggestions.

06 July 2019 3,405 9 View

Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run??

Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run?? one more thing what is the pore size of Whatmann filter paper??

03 April 2019 2,560 2 View

How do make a glycerol stock of bacteria for future use?

How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.

01 February 2019 3,626 3 View

I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?

I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?

01 February 2019 3,040 4 View

Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it?

Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity...

01 February 2019 7,864 5 View

If bacterial stock is contaminated with other bacteria, How can be sure that whether it is contaminated or not?

Everytime i found different OD data which was fluctuating and sometimes continue increase of OD .Though growth curve and colony under microscope dont tell wheather it is contaminated or not.If...

08 September 2018 461 15 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View