Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity considering cell volume.
Not equal to, but in direct proportion to the cell volume. The fluorescence intensity of GFP expressed as a cytosolic protein is directly proportional to the amount of GFP present, which in turn is directly proportional to the number of cells expressing it. The volume of cytosol is also, of course, directly proportional to the number of cells. Hence, the GFP fluorescence is directly proportional to the volume of cytoplasm of the cells expressing it.
GFP fluorescence increases in direct proportion to the GFP gene copy number delivered to cells. The intensity of GFP fluorescence is directly proportional to GFP mRNA abundance in cells. This conclusion is further supported by the fact that the induction of GFP gene expression from two inducible promoters TRE and ICP0 are readily detected by flow cytometric measurement of GFP fluorescent intensity.
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Not equal to, but in direct proportion to the cell volume. The fluorescence intensity of GFP expressed as a cytosolic protein is directly proportional to the amount of GFP present, which in turn is directly proportional to the number of cells expressing it. The volume of cytosol is also, of course, directly proportional to the number of cells. Hence, the GFP fluorescence is directly proportional to the volume of cytoplasm of the cells expressing it.
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