An insert length of 811bp (17ng/ul) and digested vector size of 5.6kb (35ng/ul).

--100ng vector amount--. Am I right in using the following respective volumes of insert and vector? 2.5ul and 2.8ul.

Using NEB's kit, I'll then add 10ul Master mix and 4.7ul of nuclease-free water to get a total volume of 20ul in the PCR tube.

Thanks a lot in advance!