I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

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Natanael Mansilla

Azra,

We use a protocole slightly modified of this paper..

Wittig I, Karas M, Schägger H (2007) High Resolution Clear Native Electrophoresis for In-gel Functional Assays and Fluorescence Studies of Membrane Protein Complexes. Mol Cell Proteomics 6: 1215–1225

We use a 3-13% gradient separation gel . I attached an excel with the recipe. The gelification takes around 20-40min at 20ºC.

I hope that it help you!

Azra Rafiq

Thankyou so much.