Azra Rafiq

2 Questions 3 Answers 0 Followers

Questions related from Azra Rafiq

I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

05 April 2019 4,894 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

26 March 2019 10,006 6 View