Since BG-11 media has no NaCl content in it, I might have to add NaCl separately. If I add NaCl externally, what concentration of NaCl should I consider as my control? Kindly guide me regarding addition of NaCl externally to BG-11 media.
For such experiments where some stress agents are used, minimal media is recommended. In such experiment the minimal media of particular microbe is used as control and same media with certain stress agents is used as treatment.