I am working on measuring ROS from erythrocytes using H2DCFDA, please what is the best medium to run the experiment?
We have used Medium199 for culturing cells and measured ROS level.. it has worked well in our system.
I use DMEM added with glutamate and pyruvate but no phenol red and FBS. Good luck.
@Ying Yang, thanks for the answer. When you used the DMEM, were there issues of artifact formation with DMEM and the probe (H2DCFDA)?
Medium199 is a better option for culturing cells and measuring ROS level. It has worked well in our laboratory.
I need to multiply the inverse of a matrix A to another matrix B. If B were to be a vector, I would simply solve a linear system Ax = B to get the product x=inv(A)B. With B being a matrix, I...
29 April 2020 10,001 12 View
Many gender or feminist journals are pro-feminist and decline to publish essays that treat gender relations without supporting feminism or which place significant emphasis on the protection and...
12 January 2019 1,759 8 View
I am working on Autonomous Learning Techniques for robots. Assuming a robot is deployed in an unknown environment, what techniques could help the robot explore this environment and build its own...
15 November 2017 6,988 3 View
Hello all; I have been able to capture an over-the-air LTE waveform and decoded both MIB and SIB1 from. I was able to convert the SIB1 bit to decimal but am unable to interpret my result. I have...
19 September 2017 5,531 2 View
19 September 2016 1,459 15 View
By this, I mean how to make an intelligent tutoring system learn a student's approach to questions and understanding based on previous experiences. Having the capacity to know at hand likely...
11 February 2016 3,493 4 View
I need the English version of this paper which was publish in 1871.
27 August 2015 5,576 2 View
I am trying to design a turbine stator with a unique internal cooling structure and I need a way to create the blade in ansys, or import it to ansys for testing. I found ansys has a bladegen...
26 February 2015 3,759 3 View
I need a well developed pre- and post-processor that can generate complex meshes and provide all the functionalities needed to solve flow problems in it. And also allow visualizations. The goal is...
11 February 2015 2,728 15 View
Given a PDE model with some constraints restricting the range of values of model quantities, how does one apply the idea of relaxation to such a model? Any idea or references on relaxation idea of...
22 September 2014 1,366 11 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View