I want to stain muscle cells for presence of glycoproteins. For this I am trying to do lectin staining. However, right now I do not have a working protocol and I have come across 2 lectins in literature that can bind N-glycans (glycoproteins i am interested in). I want to know which of these two could be a better choice? And if anyone has done lectin staining before and can suggest me a protocol or a product? I am ok to use a fluorescence conjugate lectin or a regular one with the use of secondary antibody biding.
Hi:
Our working group has performed staining with FITC-labeled lectins in larval tissue, but we have used a lectin that is not one of the ones you mention. From what I saw in the literature, there is an article that stains muscle fibers with WGA (https://doi.org/10.1002/jemt.20865) and that could be very useful. If you are not familiar with the lectin labeling technique, you could consult the reference DOI: 10.1016/j.cbpb.2022.110770.
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