Dear Sulail

It depends on your tissue type so be guided by literature 

However there are a  number that tend to be ubiquitous 

1. B actin

2. B2 microglibulin

3. B tubulin

4. gapdh

5. 18s RENA

6. Tata binding protein 

You can use b actin gene its widely use

Dear Sulail

It depends on your tissue type so be guided by literature 

However there are a  number that tend to be ubiquitous 

1. B actin

2. B2 microglibulin

3. B tubulin

4. gapdh

5. 18s RENA

6. Tata binding protein 

Dear Sulail,

I agree with Lawrence, you can use

1. GAPDH

2. Beta ACTIN

3. TATA binding protein

4. Cyclophilin

5.18S rRNA.

First check the expression of these transcripts and then select the housekeeping gene.

In my experience 18SrRNA has worked well.

Thank you.

Regards,

Prasad.

Thank you guys. Using 2 control genes are recommended, right? Dr. 

Dr. Pethe, you've used 18SrRNA for cardiac tissue (rats)?

Dear Sulail

find attached 2 x papers describing housekeeper gene selection in general and onother specifically referring to cardiomyocytes

Dear Sulail,

I have used 18S rRNA for lookig at expression of cardiac specific genes from human embryonic stem cells.

Thank you.

Regards,

Prasad.