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Questions related from Sulail Fatima
I performed gelatin zymography on cardiac tissue extracts. I was expecting mmp-2 and mmp-9 bands as reported by references. Where did I go wrong? Both attachments are same gels. Just there is the...
17 January 2018 5,252 19 View
I extracted RNA using Riboex (Geneall) followed by Trizol method. I have 2 types of tissues: Infarcted & non-infarcted cardiac tissue ( I am planning to perform RT-qPCR). I guess I am having some...
01 January 2018 7,235 16 View
It might sound lame but I am new to it. I don't get first I must optimize the primer or check for my qpcr efficiency by serially diluting my cdna. Can anyone briefly explain how I can do these...
16 December 2017 5,097 3 View
According to this, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907514/ Polr2a, Rpl32 and Tbp seem suitable for studying ventricular remodeling/heart failure in rats' cardiac tissue. Kindly share...
06 August 2017 8,744 7 View
Can anyone suggest which enzymes I shall use for preparing single cell suspension from cardiac tissue. I will be looking at both cell surface and intracellular antigens involving cardiomyocytes...
15 July 2017 4,772 3 View
I have been reading about various drugs inducing anti-remodeling effects in animal models (rats) of myocardial infarction. Can someone suggest why ischemia-reperfusion is not preferred in this...
16 March 2017 7,415 4 View
I am using Labchart 5 to record intra-carotid & intra-ventricular pressure. Can anyone suggest how can we obtain dp/dt minimum and maximum? After selecting the pressure waves during a specific...
07 March 2017 4,926 5 View
After arresting the rat's heart diastole, I freeze it for an hour and half. I use rat heart slicer to make sections (2 mm) for ttc staining. After staining, my most of the sections are wrinkled. I...
12 December 2016 653 2 View
I am studying MMP-2 and MMP-9 activity by gelatin zymography. The bands I have obtained are smeared up (within their lanes). Can anyone suggest possible reasons for this?
24 August 2016 4,942 9 View
A drug that has no anti-inflammatory and anti-oxidative effects. Furthermore, it shall not induce cardioprotection. Also that, I am working on opioid system the drug shall not be a opioid agonist....
28 April 2016 7,979 3 View
I am presenting an article in weekly journal club meeting and the authors have used " Masson's Trichrome Staining and Picrosirius red staining to asses fibrosis & collagen content,...
03 January 2016 6,891 7 View
I wish to study the effect of my drug on fibroblast differentiation into myofibroblasts. So far, I am unable to hold on to any marker that is specific for myofibroblasts (is not expressed by any...
14 October 2015 9,040 4 View
I wish to perform zymography for MMP-2 and MMP-9. I am working on cardiac remodeling and I have cardiac tissues as my sample to work on. I am confused if I shall extract protein from my tissue or...
05 October 2015 4,454 7 View
