Sulail Fatima

9 Questions 23 Answers 0 Followers

Questions related from Sulail Fatima

Why no bands in gelatin zymogram? And something unusual at the bottom of gel?

I performed gelatin zymography on cardiac tissue extracts. I was expecting mmp-2 and mmp-9 bands as reported by references. Where did I go wrong? Both attachments are same gels. Just there is the...

17 January 2018 5,247 19 View

I checked RNA conc. using nanodrop. Can anyone suggest what the peaks are speaking about?

I extracted RNA using Riboex (Geneall) followed by Trizol method. I have 2 types of tissues: Infarcted & non-infarcted cardiac tissue ( I am planning to perform RT-qPCR). I guess I am having some...

01 January 2018 7,226 16 View

Which one I shall do first, qpcr efficiency and primer optimization?

It might sound lame but I am new to it. I don't get first I must optimize the primer or check for my qpcr efficiency by serially diluting my cdna. Can anyone briefly explain how I can do these...

16 December 2017 5,092 3 View

I am looking for stable reference genes for RT-qPCR. If anyone can suggest?

According to this, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907514/ Polr2a, Rpl32 and Tbp seem suitable for studying ventricular remodeling/heart failure in rats' cardiac tissue. Kindly share...

06 August 2017 8,740 7 View

Enzymes for preparing single cell suspension from cardiac tissue for flow cytometry?

Can anyone suggest which enzymes I shall use for preparing single cell suspension from cardiac tissue. I will be looking at both cell surface and intracellular antigens involving cardiomyocytes...

15 July 2017 4,767 3 View

Why permanent ligation of coronary artery is more preferred for studying anti-remodeling effects of drugs in regards to myocardial infarction?

I have been reading about various drugs inducing anti-remodeling effects in animal models (rats) of myocardial infarction. Can someone suggest why ischemia-reperfusion is not preferred in this...

16 March 2017 7,400 4 View

How can I prevent heart sections from being wrinkled?

After arresting the rat's heart diastole, I freeze it for an hour and half. I use rat heart slicer to make sections (2 mm) for ttc staining. After staining, my most of the sections are wrinkled. I...

12 December 2016 649 2 View

Why my SDS-PAGE gel is getting smeared?

I am studying MMP-2 and MMP-9 activity by gelatin zymography. The bands I have obtained are smeared up (within their lanes). Can anyone suggest possible reasons for this?

24 August 2016 4,938 9 View

What is the difference between Masson's Trichrome Staining and Picrosirius red staining?

I am presenting an article in weekly journal club meeting and the authors have used " Masson's Trichrome Staining and Picrosirius red staining to asses fibrosis & collagen content,...

03 January 2016 6,887 7 View