I am having problem with my EMSA expt. My shifted band is very fuzzy. I have tested buffers, glycerol, protein, dIdC and DNA conc. as well. Any idea ?

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Mukesh Maharjan

Yes I have made all the reagents and buffers new and tried again. I also tried different glycerol concentrations.

Or do you have particular suggestion in gel prep ?

Johnson Luwang Wahengbam

Hi Mukesh,

First question: Have you checked the quality of Protein(X) and Oligos before you set up the experiment? You should have checked whether the protein sample is homogeneous or Oligos sample is pure! then go for the next step. There might be possibility of contamination in your sample.