Mukesh Maharjan

5 Questions 6 Answers 0 Followers

Questions related from Mukesh Maharjan

Problem with Forskolin Induced swelling (FIS) assay of organoids ?

Hi all, I am doing Forskolin Induced Swelling (FIS) assay. It used to work very much fine a few months ago and all of sudden it has stopped working (or instead of swelling it shrinks now !). I...

08 March 2021 7,628 1 View

Which genes are good to test activated microglia in vitro by qPCR ?

I am very new to the field of AD. I was searching for a few candidate genes in the literature that I can check by qPCR and immunoblot to show Microglia activation from microglia culture. I have...

14 January 2020 5,483 5 View

Genotyping of 5xFAD mouse, not reliable PCR results ?

I have been trying to genotype my 5xFAD mouse ordered from Jackson lab. For PCR, I use tail samples and Primers ordered from Jax website for those mice. I am not getting consistant results....

26 November 2019 2,130 3 View

Is Lipofectamine 3000 good with serum and antibiotics for Drosophila S2 cells ?

Hi all, I am transfecting Drosophila S2 cells for Luciferase assay. My cells are in 24 well plates, in 1ml S2 complete media (10% FBS + antibiotics). Is it okay if I just add DNA-Lipid complex...

15 May 2019 6,578 2 View

I am having problem with my EMSA expt. My shifted band is very fuzzy. I have tested buffers, glycerol, protein, dIdC and DNA conc. as well. Any idea ?

Even my free DNA seems not sharp. I do not know what else to try. Even though I repeated exact same conditions from my previous grad student I am not being able to replicate the result. I have the...

26 July 2018 6,700 3 View