I am getting multimeric band in untreated glutaraldehyde recombinant protein in glutaraldebhyde crosslinking why ?

08 August 2025 1 6K Report

I was doing glutaraldehyde crosslinking experiment of recombinant protein , i am trying to crosslink at room temerature but I am getting more than one band in untreated glutaraldehyde

Omar Gonzalez-Ortega

I assume you are analyzing the results in SDS-PAGE. Are you using reducing conditions? What reducing agent are you using? Does your recombinant protein have cysteine residues?

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