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Questions related from Akhilesh kumar Gupta
I was doing glutaraldehyde crosslinking experiment of recombinant protein , i am trying to crosslink at room temerature but I am getting more than one band in untreated glutaraldehyde
08 August 2025 6,375 1 View
I am working on drug target, I have to do spectrophotometry enzyme assay and enzyme kinetics of this protein in 260nm. So I don't have uv transparent plate. So, can we use clear bottom Corning...
22 February 2025 8,674 1 View
After purification,my protein getting precipitated. for Cell lysis, equilibriation , wash buffer and elution buffer , used 20 mM tris HCL of pH 7.8. I set up dialysis in different percent of...
21 October 2024 4,979 1 View
Asper discussion, First time my clonning was failed becuase the first 45 nucleotide was not cloned in pet30 a vector then I choosed the different clone of 1:1 ration fraction, I got the size of...
14 June 2024 7,678 7 View
NdeI restriction site was added in forward primer and HindIII was added in reverse primer size of band is looking near 500 bp but size of is less than 15 kda but expected size should be 17.7kda.
16 April 2024 251 5 View
please anyone tell me why this happens
01 January 1970 6,298 2 View