How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How does GROMACS calculate the average structure of proteins?

I want to get a average structure from a pdb file containing multiple models. I used the command below: gmx cluster -f input.pdb -s topol.tpr -cutoff 20 -method gromos -av GROMACS...

25 February 2021 6,314 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

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The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

R/Python package for time-intensity or progressive profiling in sensory evaluation?

Hi I've been looking for a stat package for my sensory evaluation data (progressive profiled which is a multi-timepoint version of descriptive analysis). Is there any available one to...

08 February 2021 9,170 3 View

How can I make sure the simulation results of the water droplet impacting on the wall using the FLUENT agree with the experimental results?

Dear researchers, I am modeling the process of the water droplet impacting on the wall with a constant contact angle. The specific calculation parameters are as follows: The droplet diameter is...

24 January 2021 1,020 5 View

How to see the expression of 6kda protein?

Dear all, I am now investigating the expression of small size protein (6 kda). However, the expression of the protein cannot be shown clearly after performing western blot. Therefore, are there...

13 January 2021 3,121 3 View

Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

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can anyone know the range of the osteoclast activity range (resorption) in bone remodeling cycle in mice compared to humans. or any paper talking about this information. I need this specific...

14 September 2020 1,218 2 View

Can anyone suggest why my section become black after drying for some minutes?

I stained a bone tissue with TRAP and counterstained with Mayers hematoxylin for 5 seconds. After rinse in running tap water, the result was just fine. However after I dried the section for some...

09 October 2019 1,814 1 View

Coculture of T cells and Osteoclast precursors?

Hi, I am trying to set a co-culture system of T cells from immunenized mice and mcsf treated mouse bone marrow. However, it is not successful to induce osteoclasts. I read that we can fix...

24 July 2019 7,157 5 View

Coculture of T cells and Osteoclast precursors?

Hi, I am trying to set a co-culture system of T cells from immunenized mice and mcsf treated mouse bone marrow. However, it is not successful to induce osteoclasts. I read that we can fix...

06 July 2019 5,036 4 View

Why an actin band does not appear on gel after PCR for BMM diffrerentiated into osteoclasts?

Hello everyone, I am currently trying to set up at a new lab in order to begin experiments on osteoclasts. Before starting anything, I wanted to make sure that BMMs have indeed differentiated...

17 May 2018 3,308 3 View

Why an actin band does not appear on gel after PCR for BMM diffrerentiated into osteoclasts?

Hello everyone, I am currently trying to set up at a new lab in order to begin experiments on osteoclasts. Before starting anything, I wanted to make sure that BMMs have indeed differentiated...

04 May 2018 4,036 4 View

Are there known chemotherapeutic drugs with the positive indirect effect in the case of the treatment of multiple myeloma ?

For example, I mean drugs that indirectly reduce the tumoral cell population (i) by influencing paracrine interactions between osteoblasts and osteoclasts... (ii) by influencing (solely)...

03 April 2018 8,204 2 View

Any advice on TRAP staining mouse femurs?

We are TRAP staining mouse femurs, and eventhough the osteoclast staining looks specific they are staining yellow instead of red. Any thoughts?

02 March 2018 4,366 3 View

U937 differentiation into osteoclast-like cells?

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02 March 2018 8,807 1 View