28 May 2023 1 9K Report

What is the best conserved sequence to identify actinobacteria?

More Karen Nohemí Benalcázar Andrango's questions See All
What are the considerations for creating a community of practice for ESL students?

how do i set one up? What do I include?

22 July 2024 6,097 4 View

Why and how to solve unmatched forward and reverse sequencing result by Sanger sequencing?

I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...

01 July 2024 8,328 5 View

Attempting an antibody titration?

Is it ok to titrate new antibodies at a low cell concentration like 100,000 cells per 100 uL instead of 1x10^6 cells per 100 uL? I want to titrate four new antibodies at 4-5 different dilutions to...

02 May 2024 7,613 1 View

How can I correct an error in ResearchGate?

They say we were cited by an article but we weren't. "Karen, we found a recent article that cited you:Relationship of serum total cholesterol and triglyceride with risk of mortality in...

15 April 2024 3,993 0 View

How much PCR product to load on agarose gel?

I have the PCR products of two bacterial genes (gyrA=1024 bp and rpoB=808 bp) and I want to know how many microliters I should add in the agarose gel?.

27 March 2024 2,798 7 View

Thermo Xcalibur error who can help to solve?

All working GC Thermo 1310 simple configuration GC FID in first line. Last week error apear when starting the program that Thermo aqusition service not runing. Reinstalation of Win 10 operation...

06 March 2024 6,701 8 View

I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?

I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?

30 December 2023 7,677 0 View

What are the effective practices and challenges of teachers in obtaining the TPACK?

Teachers are expected to gain knowledge in pedagogical, content and technological aspects to be effective in the field of teaching. Yet, there are teachers who have a hard struggle to become fully...

06 October 2023 7,580 2 View

Is it possible to mix bacteriological agar with PDA culture medium of any brand?

Hello everyone, I am doing my undergraduate thesis and I need to make medium strength PDA culture medium. For this, I must mix 19.5 g/l of PDA culture medium plus 7.5 g/l of Bacteriological agar....

20 September 2023 7,923 0 View

General Linear Model or Mixed Model?

Hi, My current research question is looking at whether children at 5-8 months of age have memory. To do this, we have run an eye-tracking study doing 3 encoding slides and 1 test slide. in the...

13 September 2023 3,335 1 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View

How many samples do I need, which agar and DNA sequencing technique is most preferable when carrying out microbial diversity in three water bodies?

I am planning to conduct a research project on the microbial diversity in three different water bodies and I would like to know the optimal number of times I should sample each water body to...

06 June 2024 9,946 1 View

I did whole genome sequence for a leishmania parasite I got the sequence of rDNA in stead of GDNA can anyone ?

my question about the sequence analysis I did whole genome sequence for a leishmania parasite I got the sequence of rDNA in stead of GDNA can anyone ?

30 May 2024 8,671 0 View

How can I identify an unknown DNA sequence & termination ?

Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you

26 May 2024 1,590 3 View

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