Karen Nohemí Benalcázar Andrango

6 Questions 1 Answers 0 Followers

Questions related from Karen Nohemí Benalcázar Andrango

How much PCR product to load on agarose gel?

I have the PCR products of two bacterial genes (gyrA=1024 bp and rpoB=808 bp) and I want to know how many microliters I should add in the agarose gel?.

28 March 2024 2,781 7 View

Is it possible to mix bacteriological agar with PDA culture medium of any brand?

Hello everyone, I am doing my undergraduate thesis and I need to make medium strength PDA culture medium. For this, I must mix 19.5 g/l of PDA culture medium plus 7.5 g/l of Bacteriological agar....

21 September 2023 7,904 0 View

Is it advisable to use bacterial or actinomycete primers to perform molecular identification?

The bacterial strains I have grew on actinomycete agar and I need to identify them.

27 July 2023 1,767 3 View

To identify if my bacterial strains are from acinomycetes, should I use primers for 16S rRNA from eubacteria or actinobacteria?

I am carrying out a project of molecular identification of strains that are possibly actinomycetes. However, I am not sure if the primers I should use are for eubacteria or actinobacteria, since...

26 July 2023 6,378 3 View

What kind of kit can I use to extract DNA from actinobacteria?

Good morning everyone! I am a Biotechnology student and I am doing a project to verify the biocontrol capacity of actinobacteria, for this I must carry out the molecular identification of the...

28 May 2023 5,536 3 View

I am doing my undergraduate thesis, can someone help me?

What is the best conserved sequence to identify actinobacteria?

28 May 2023 8,715 1 View