I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?
I am working with the NL-G-F and 5x FAD mice. Are there any sequencing databases publicly available to mine for brain or retina? Thanks!
20 January 2021 6,425 1 View
For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...
11 January 2021 6,016 3 View
How do you use Remote sensing and GIS in transportation, what are the types of images you will require, what will be their wavelength, what type of sensors will you require?
20 December 2020 6,375 5 View
Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...
18 September 2020 5,894 3 View
I am analysing treatment group differences at time 2 (two time points only) data on a psych measure outcome (with five covariates: age, gender, CVD, BMI and F2s), and I am interested in one of the...
25 August 2020 6,904 3 View
Hello! Does anyone has a effective way to autoclave 10% skimmed milk without caramelization? I tried 121C for 15min (in the powder form) but I am struggling with the color which is undesirable....
30 June 2020 4,601 3 View
Hi everyone, I am coating stainless steel with 10%PLA and Nanoparticles, and I am trying to find out techniques that I can use to characterize the coating surface aside from SEM and to quantify...
08 June 2020 2,293 7 View
We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is...
28 April 2020 4,704 3 View
I am trying to do for my thesis a uniform homogeneous coating with PLA (polylactic acid with 1.05 dl/ g viscosity) in Stainless steel 316L medical grade via spin coating, but I haven't been able...
07 April 2020 8,556 3 View
I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in...
28 March 2020 1,559 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...
01 March 2021 2,294 3 View
I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...
23 February 2021 5,503 1 View
Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...
18 February 2021 190 3 View
Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...
18 February 2021 3,223 3 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
My study consists of a conceptual framework with seven constructs measured in seven point Likert scale. According to pilot study, only three constructs have Cronbach alpha value more than 0.9...
12 February 2021 9,368 2 View
Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...
08 February 2021 9,671 3 View
I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...
06 February 2021 336 3 View
I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...
31 January 2021 4,285 3 View