DNA pellet in wash buffer seems different in my experience. Is this a DNA molecule or something else?
It's going to be a mixture of DNA and RNA both unless you treated with an RNase enzyme.
Also, depending on the "dirtiness" of the protocol (e.g. if you used boiling lysis instead of a spin column), you'll also have a lot of cell debris.
Dear Katie A S Burnette
pellet contains DNA, you should be able to proceed with further purification or analysis. If you are still unsure, consulting the specific DNA isolation protocol.
Thank you
Regards,
Lenin Kumar B
Lenin Kumar Bompalli it was not my question. Shivansh Negi posted a picture of the pellet from their DNA extraction & wanted to know what was in the pellet.
Shivansh, did you try measuring the purity & concentration with a spectrophotometer? That can help you determine what you got.
Good luck!
Thank you for the answers of all the respondends. I will remember your suggestions while trying next time.Thank you again.
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