Hybridoma technology: What is the common B cell differentiation stage used for hybridoma technology?

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Lora Benoit

The resultant hybridoma cells are selected on the basis that they are secreting antibodies. Since the myeloma fusion partner is unable to secrete antibodies, this means that the other cell must be a plasma cell.

Ishak Ozel Tekin

Early B cell stage is not useful as a fusion partner on hybridoma technology. Actually please do not prefer naive mature B cell ..You have to wait Ig class switching after immunization for good antibody production (Ig class switching needs T cell help). If you use early stage B cell (immature or naive mature B cell), yo can get just low affinity IgM.

Sincerely

IShak