22 Questions 8 Answers 0 Followers
Questions related from Nav Hh
Hi, I was curious to know what factors affect the resolution of a quadrupole mass analyzer. I find a lot of electrical settings that may affect the mass of the ions that are being transmitted. Is...
18 January 2021 6,128 1 View
Hi, What is the common B cell differentiation stage used for hybridoma technology? I understand that these are antibody producing B cells upon the injection of antigen, but do people use mature B...
29 September 2020 1,954 2 View
is it possible that mass spectrometry can tell radical ions or is it not not possible? (Because loss of an electron will result in only 0.0005 Da, there is no instrument with such high...
13 August 2020 7,198 1 View
Hi all, My understanding about the grid used in ToF is it is useful to reduce the broadening due to the varied kinetic energy of the ion. But also it may reduce transmission (90% or less). Is...
08 August 2020 9,632 0 View
Resolving power of TOF increases with longer drift time (mass/delt(mass) = (t/delt(t) using the kinetic energy ((1/2)*mv^2) and potential energy(z*e*V) ). t is the centroid of drift time. delt(t)...
28 September 2018 9,655 6 View
How do people study the oxidation state of divalent metal ions? The difference is number of electrons, which are very small in mass. How do people actually study this experimentally?
10 August 2018 8,144 3 View
Hi, I am curious to find out what are some meaningful difference between ADC and TDC detection (analog and time digital converter). What are some scenarios where one chooses one of the detection...
10 August 2018 2,296 2 View
Hi, Does freeze-thaw cycle still affect the protein stability despite flash freezing with liquid nitrogen? Thanks!
07 July 2017 5,831 4 View
Hi all, This question may solicit some practical ways of analyzing MS/MS data. I am performing collision induced dissociation to fragment my protein. The goal of my experiment is to observe the...
05 June 2017 4,398 4 View
Hi all, this might be a silly question. But I am wondering if there are solvent molecules in the core of a protein where hydrophobic interactions are primary. We all know that water or general...
18 April 2017 5,694 3 View
Hi all, Just a quick question. I know typically to allow positive ion mode in MS alone, people use acidic buffer and for negative ion mode basic buffer. When coupling LC and MS, how do we induce...
20 February 2017 8,557 6 View
Hi all, Many commercially available MS has "positive" and "negative" ion mode even the ones that use ESI. What is the mechanism for these? (by mechanism I mean electron impact ionization or or...
20 February 2017 1,101 1 View
Hi all, A quick question for structural biologists, how do we determine if a region is hydrophobic or hydrophilic region? Is there a measurement that we can do once the structure is available? or...
30 January 2017 2,430 6 View
Hi all, For some reason, I can't find a bcr-abl tyrosine kinase for the life of me. I am looking for just a simple structure of bcr-abl tyrosine kinase (active state). Does anyone know a PDB for...
26 January 2017 2,595 3 View
Hi all, Just had a quick question about for most of the buffer system, why is making buffer at pH pKa±1 is recommended? What is the reason behind it? I've seen people violating this rule all the...
14 January 2017 6,760 6 View
Hi all, I am learning a lot of things about the computational biology for the first time. I was curious, with the current technology, what is the possible simulation time range for a research in...
02 January 2017 8,018 4 View
Hi all, Typically, in a generic ligand binding equation, [L]+[R] -> [LR], the fraction bound receptors ([LR]/([L]+[LR]) can be written as [L]/(Kd+[L]). From this when we plot, fraction bound...
13 December 2016 4,602 4 View
Hi all, I want to understand the heat changes that come from anions (PO4-) interacting with metal ions (Cu^2+). PO4- is supposed to interact with metal ions very strongly as it is used to...
12 December 2016 1,825 5 View
Hi all, My name is Seoyeon. I am preparing metal binding experiment to a protein of interest. It was previously shown that zinc binds to the protein. For some reason I can't tell that the metal...
08 July 2016 2,086 2 View
Hi all, I am running my protein sample using mass spectrometry.. I noticed that there are sodium adducts in my protein sample. I want to reduce the amount of sodium ions in my sample. I was told...
06 May 2016 1,940 4 View
Hi all, In the field of antibody, I hear a lot about orphan antibody. Are these orphan antibodies simply antibodies that exist but without purpose (e.g., no evident clinical benefits associated...
01 January 1970 8,645 4 View
Hi all, Monoclonal antibody biosimilars are the monoclonal antibody produced from the same standards of pharmacutical quality as the original monoclonal antibody when the patent of the original...
01 January 1970 5,307 2 View
