Tissue Dissociators for mouse spleens and lymph nodes?

18 January 2021 6,804 6 View

Automated Cell Counters

Hi all. I'm wondering what everyone's thoughts are on automated cell counters for use with mouse lymphocytes. I have used the Countess II FL in the past, but found it to be less than ideal as it...

01 January 1970 9,063 2 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How to increase hybridoma viability?

I am culturing a murine b cell hybridoma in RPMI with 10% FBS, 1% pen/strep and 1% L-Glutamine. However when I count them on a haemocytometer with trypan blue the vast majority appear dead. I have...

11 December 2019 1,176 3 View

How can I visualize hybridoma formation or phagocytosis?

I'm trying to determine if two cell types are merging, possibly through phagocytosis, to explain some data I've been seeing. Can anyone recommend an assay or technique that would allow me to...

07 August 2019 378 3 View

Could any monoclonal antibody be a capture antibody?

Dear all, I have made an monoclonal antibody by using hybridoma technique. The antigen is a KLH-peptide and I did the screening using full-length protein (direct ELISA). After selecting a positive...

06 July 2019 9,340 3 View

Is there a supplement I can add to hybridoma cell culture to encourage disulfide bond formation and prevent partially reduced IgG1 artifacts?

I have been purifying IgG1s from hybridoma culture supernatants. These hybridomas have been producing partially reduced artifacts, the most common being IgG1 missing a single light chain (HHL)...

04 May 2019 3,143 1 View

Is there a supplement I can add to hybridoma cell culture to encourage disulfide bond formation and prevent partially reduced IgG1 artifacts?

I have been purifying IgG1s from hybridoma culture supernatants. These hybridomas have been producing partially reduced artifacts, the most common being IgG1 missing a single light chain (HHL)...

01 May 2019 7,369 1 View

What is the relation between pH and dissolved oxygen in a bioreactor cell culture?

I am currently working on a perfusion culture of hybridoma. I do not know how pH may has an effect on dissolved oxygen and glucose. We are using NaHCO3 for base adition, and it looks like is...

03 April 2019 3,465 4 View

Can I use a mouse anti human flow cytometry antibody for western blot?

Can I use a purified mouse anti human cd63 that is for flow cytometry for western blot?...

20 February 2019 2,485 6 View