Hi there, I'm researching various methods of tissue dissociation of mouse spleens and lymph nodes. I've been doing it manually for decades, but I know there are machines out there that supposedly work quite well. I'm trying to find evidence of how successful these machines actually are. One in particular that I've been looking into is the Bullet Blender from Next Advance. Unfortunately, the beads and tubes are not sterile, so I would have to autoclave them which is an added step that takes time. Does anyone out there have firsthand experience with any homogenizer machines that are currently on the market? FYI, I tried the gentleMAC Tissue Dissociator from Miltenyi Biotec a few years ago, but it wasn't as good as my manual method.
Thanks in advance.
The reason the Bullet Blenders are uniquely appropriate for tissue dissociation is that you can lower the speed so much. On most bead mill homogenizers, the minimum speed is relatively high, meaning that you will end up homogenizing most of your cells.
https://homogenizers.net/collections/bead-mill-homogenizers
FYI, no homogenizer will reliably produce results as good as a gentleMACS when it comes to cell isolation / tissue dissociation, as the gentleMACS is specifically designed for tissue dissociation. Homogenizers will almost always have a lower yield of viable cells. The comparative upside to a Bullet Blender is the much lower price and higher throughput. If your manual method worked better than the gentleMACS and you are going to prioritize yield and viability above all else, then I would suggest you stick with your current manual method.
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