Joanne Smith

6 Questions 4 Answers 0 Followers

Questions related from Joanne Smith

Does anyone know how soon after mouse death, lymph nodes should be harvested for lymphocyte isolation?

Just wondering if there is a maximum time limit before lymphocyte viability is severely hindered after mouse death. For example, sometimes an immunization can result in a negative outcome (i.e....

24 July 2025 747 2 View

Low Antibody Yield after purification of Bioreactor Harvests?

Hi there. I inoculated a CL1000 bioreactor with a hybridoma cell line and cultured it for 6-8 weeks. After purification, the antibody concentration was lower than expected. We have previously used...

07 November 2023 7,710 0 View

Hybridoma cell diameter?

Does anyone know what the diameter of a mouse hybridoma cell is? I can't find any information online and I'm curious. I've read that the average diameter of a mouse lymphocyte is 5-10um and the...

15 December 2021 6,833 1 View

Tissue Dissociators for mouse spleens and lymph nodes?

Hi All, I'm curious if any of you have used this model of tissue dissociator for mouse spleens or lymph nodes before? gentleMACS™ Octo Dissociator

19 January 2021 7,034 6 View

Automated Cell Counters

Hi all. I'm wondering what everyone's thoughts are on automated cell counters for use with mouse lymphocytes. I have used the Countess II FL in the past, but found it to be less than ideal as it...

01 January 1970 9,225 2 View

Bullet Blender success for dissociation of mouse spleens and lymph nodes

Hi there, I'm researching various methods of tissue dissociation of mouse spleens and lymph nodes. I've been doing it manually for decades, but I know there are machines out there that supposedly...

01 January 1970 8,921 1 View