How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Adding xanthan gum to aid particle suspension during laser particle size analysis?

Can you add a thickener or viscosity modifier such as xanthan gum or carboxymethyl cellulose into your sample when performing laser particle size analysis to help keep particulate samples in...

06 November 2020 6,790 7 View

What is the best washing procedure for Gelatin - arabic gum capsules?

I am preparing microcapsules from complex coacervation procedure to encapsulate an oil phase. However, filtration to remove the aqueous phase results in a paste of aggregated particles. So I am...

25 October 2020 3,152 3 View

Possible nanoaprticle synthesis molecular dynamics simulation?

I would like to simulate the formation of polymer nano-complex using two biopolymers and find the effect of the concentration of both the substrates on the particle size of that nano-complex in a...

02 October 2020 9,887 1 View

Can you explain more experiences regarding research about "Guar Gum and oil emulsions" ?

Dear all, I would like to know about guar gum as coating material for oil encapsulation. Please Suggest...? Need to find research experiences under Guar gum.....

19 July 2020 4,810 2 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How to increase hybridoma viability?

I am culturing a murine b cell hybridoma in RPMI with 10% FBS, 1% pen/strep and 1% L-Glutamine. However when I count them on a haemocytometer with trypan blue the vast majority appear dead. I have...

11 December 2019 1,176 3 View

Carboxylate ion IR bands can appear in solid state?

I got a IR spectrum of Gum Arabic and two bands appear around 1600 and 1400 cm-1 respectively. I found these bands are related to carboxylate ion but I got the spectrum of the Gum Arabic powder....

16 August 2019 1,391 3 View