02 February 2024 1 3K Report

Can I use gellan gum to solidify growth media for hybridoma clonal selection?

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Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,718 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,166 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,676 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,197 5 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 475 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,228 0 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,288 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,829 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,159 0 View

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How is surfactant addition calculated?

I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...

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What is the basic difference between gum and hydrocolloid?

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Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

The name of my thesis is: "Dental Status and Oral Hygiene of Dialysis Patients in Opava Region". Should I replace the word "of" with "in"? Thank you?

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08 June 2024 2,219 7 View

Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?

Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?

20 May 2024 8,214 2 View

How can we conduct tests using p-nitrophenyl substrates?

How to establish the calibration curve of PNP in relation to concentrations in a microplate and for measuring the activity of lipase, what do we use? Substrate stock: 20 mM p-NP palmitate in...

19 May 2024 1,286 0 View

How to resue a hybridoma cell line?

Hi all We imported a tube of hybridoma cell line, but the courier company accidentally forgot to top up dry ice, while my cells were kept at -10 degree for approx 2 days. I tried to put the cells...

17 April 2024 3,128 2 View

Does anyone have B3Z T cell hybridoma?

I need B3Z T cell hybridoma in my experiment. It is not commercially available. Can someone share some with me? Thanks very much!

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Does anyone knows an alternative or alternative source to OPI Media Supplement for hybridoma growth?

The product was previously available at Millipore Sigma, but for the past six months we have not been able to order it.

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