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Questions related from Binbin Lee
What is the detection principle? In a protease activity assay, how can I determine whether the background fluorescence of the Gly-Pro-AMC substrate is significant?
last month 10,031 1 View
I'm currently exploring esterase detection methods and have been using 5-bromo-4-chloroindoxyl acetate (BCDA) as a substrate recently. I'm curious about the underlying principle—could someone...
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How does DAPI stain the cell nucleus? Are there other dyes that can stain the cell nucleus? Are there any other live cell dyes?
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When detecting p53 in human samples by Western Blot, the antibody is supposed to show a single band around 53KDa. However, I consistently see extra bands (non-specific signals).
last month 8,647 1 View
I noticed an interesting discrepancy between the results of two assays: Flow cytometry-based cell cycle analysis comparing the potency of two drugs. The results showed that drug 1 induced a higher...
22 July 2025 147 1 View
I bought the human leucine-rich α2 glycoprotein 1 (LRG1) ELISA test kit (purchased from Jianglai, JL14662) to detect the content of LRG1 protein (purchased from MedChemExpress, HY-P7016). However,...
21 July 2025 604 1 View
I am working with THP-1 macrophages and bacterial cells, specifically trying to detach THP-1 differentiated macrophages (differentiated using PMA) from tissue culture plates for FACS analysis....
18 July 2025 1,697 3 View
I used the aging staining kit but the staining was not successful and there were a lot of crystals. What is the reason? My positive control group was not stained successfully either. The x-gal...
17 July 2025 5,043 1 View
I am doing an experiment to monitor the pH of lysosomes in cells and want to try the probe CypHer 5. But there are some questions: First, is it a live cell dye? After all, we want to track the...
15 July 2025 4,600 1 View
May I ask everyone, what molecular weight of poly-lysine is generally chosen for promoting cell adhesion growth?
04 July 2025 7,206 4 View
Dear colleagues, I need to prepare a 0.5-10 mM 3-methyladenine (3-MA) solution. How should the corresponding stock solution be prepared? If friends with experience can provide suggestions, it...
03 July 2025 2,083 1 View
Where can I buy LL-37, an antimicrobial peptide labeled with fluorescein isothiocyanate (FITC)? If anyone who has used related products can share reliable channels or supplier information, I...
03 July 2025 3,516 2 View
I bought LP17 (Cat.No.: HY-P3400) from MCE. Their instructions recommend dissolving it with H₂O, adjusting the pH with HCl and ultrasonic treatment. How should I do it specifically? I also found...
30 June 2025 3,249 3 View
Dear scientific researchers, I am doing confocal imaging experiments recently and want to stain live and dead cells, but I haven't found a particularly suitable dye yet. I know there are many...
27 June 2025 8,557 3 View
