21 Questions 4 Answers 0 Followers
Questions related from Binbin Lee
What is the detection principle? In a protease activity assay, how can I determine whether the background fluorescence of the Gly-Pro-AMC substrate is significant?
08 August 2025 10,065 1 View
I'm currently exploring esterase detection methods and have been using 5-bromo-4-chloroindoxyl acetate (BCDA) as a substrate recently. I'm curious about the underlying principle—could someone...
07 August 2025 6,094 1 View
How does DAPI stain the cell nucleus? Are there other dyes that can stain the cell nucleus? Are there any other live cell dyes?
05 August 2025 1,233 3 View
When detecting p53 in human samples by Western Blot, the antibody is supposed to show a single band around 53KDa. However, I consistently see extra bands (non-specific signals).
04 August 2025 8,672 1 View
I'm planning to detect lipid-related signals on paraffin-embedded tissue sections and have the BODIPY 558/568 C12 lipid probe. I've found that it's commonly used for lipid droplet labeling in live...
01 August 2025 7,872 1 View
I'm using Tanshinone IIA (Dan Shen ketone) for cell experiments. Since I only need a small amount for each experiment, weighing the powder and preparing a fresh solution each time is impractical....
30 July 2025 9,584 1 View
I noticed an interesting discrepancy between the results of two assays: Flow cytometry-based cell cycle analysis comparing the potency of two drugs. The results showed that drug 1 induced a higher...
22 July 2025 169 1 View
I bought the human leucine-rich α2 glycoprotein 1 (LRG1) ELISA test kit (purchased from Jianglai, JL14662) to detect the content of LRG1 protein (purchased from MedChemExpress, HY-P7016). However,...
21 July 2025 625 1 View
I am working with THP-1 macrophages and bacterial cells, specifically trying to detach THP-1 differentiated macrophages (differentiated using PMA) from tissue culture plates for FACS analysis....
18 July 2025 1,718 3 View
I used the aging staining kit but the staining was not successful and there were a lot of crystals. What is the reason? My positive control group was not stained successfully either. The x-gal...
17 July 2025 5,068 1 View
I am doing an experiment to monitor the pH of lysosomes in cells and want to try the probe CypHer 5. But there are some questions: First, is it a live cell dye? After all, we want to track the...
15 July 2025 4,618 1 View
I bought a high-concentration basement membrane matrix gel containing phenol red, and it arrived in a frozen state. I'm not sure how to store it properly after receiving it, and I also want to...
14 July 2025 2,262 1 View
Has anyone worked with Ac4ManNAz? I’m wondering if it’s classified as an azide compound and whether it poses any explosion risks. Would really appreciate some insights!!
11 July 2025 8,578 1 View
I recently purchased MCE's siRNA kit and encountered two questions in the experimental design. I would like to ask you: The experiment plans to use a negative control siRNA labeled with FAM. In...
10 July 2025 8,507 1 View
I recently used the fluorescent dye DiBAC4(3) in a cell experiment. When I adjusted the voltage, I always felt that the brightness of the fluorescence seemed to change. What is the principle of...
07 July 2025 5,770 1 View
May I ask everyone, what molecular weight of poly-lysine is generally chosen for promoting cell adhesion growth?
04 July 2025 7,232 4 View
Dear colleagues, I need to prepare a 0.5-10 mM 3-methyladenine (3-MA) solution. How should the corresponding stock solution be prepared? If friends with experience can provide suggestions, it...
03 July 2025 2,097 1 View
Where can I buy LL-37, an antimicrobial peptide labeled with fluorescein isothiocyanate (FITC)? If anyone who has used related products can share reliable channels or supplier information, I...
03 July 2025 3,537 2 View
I bought LP17 (Cat.No.: HY-P3400) from MCE. Their instructions recommend dissolving it with H₂O, adjusting the pH with HCl and ultrasonic treatment. How should I do it specifically? I also found...
30 June 2025 3,261 3 View
Dear scientific researchers, I am doing confocal imaging experiments recently and want to stain live and dead cells, but I haven't found a particularly suitable dye yet. I know there are many...
27 June 2025 8,575 3 View
My research target has high sequence homology in humans and model organisms (such as mice/rat), but I am not sure whether the existing small molecule inhibitors can effectively bind and work in...
27 June 2025 3,542 1 View
