Hsa-miR-4454+hsa-miR-7975 in NanoString ( microRNA)?

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Yin Zhe

DNA is a one-time execution sequence module, and other RNAs are timing execution modules. More important than sequencing is to determine the position of the execution timer.

Sequencing is the quaternary data, A, G, is the quaternary, T is the binary C = 0.

Frederic Lepretre

Nanostring results are given for the hybridization counts of probes targeting your genes, it's a "real-time" like technology without any amplification, means no bias. on the other hand sequences (I guess you mean RNAseq) meet lot of bias in selection, purification and amplification (especially for short sequences as mir). you can't compare.

not surprising

fred

Ali Hashemi Gheinani

Hi Fred,

Thanks for your response. But question is about one of the probes in microRNA cartridge of NanoString. The prob name is hsa-miR-4454+hsa-miR-7975. this prob gives high read count. But no other platform confirms this abundance. no matter what you load in the chip, hsa-miR-4454+hsa-miR-7975 gives high read counts. How one can interpret this?

Ali

Hi Ali

ok, then take a look at the USB device sended with the panel. You should have an xls file with the panel gene list and sequences of the target. if no ask your commercial. does this sequence match the miR-7975 sequence?

Ulrich Baumgartner

Sorry but none of these answers meet the question.

Why hsa-miR-4454+hsa-miR-7975 in NanoString gives rise to high counts. However, if you perform RTqPCR or miRNASeq this microRNA is not detectable.

Sorry, Ali but I do not have an answer yet.

Miguel Ruiz Cruz

Hi Ali,

Did you find the answer of the question? And also, why did this probe (and also a few more) has a composed name instead of being two independent miRNAs target by two independent probes?

Thanks in advance