Which brands of HPLC is more robust and better-Shimadzu (LC-20AT semi-prep or Agilent (1260 Infinity II)? I am thinking to get one extra and your suggestions will be appreciated.
The answer depends on what you will be analysing and your expected concentration. Shimadzu is cheaper...
As I see a semi-prep system is being considered, please consider the ACCQPrep 125 system. It is designed for preparative HPLC rather than analytical.
For system specifications, please see: http://www.teledyneisco.com/accqprep
The dealer in Australia is:
DKSH AUSTRALIA PTY. LTD.
Phone: 1800 010 114
Fax: +61 3 9554 6677
Email: [email protected]
URL: WWW.DKSH.COM.AU/RESEARCH
Disclaimer- I work for Teledyne Isco, and mention the ACCQPrep system in response to the question about an LC system purchase.
I would suggest that you first evaluate what your actual needs are. The two systems come in many different versions so no comparison can be or has been made, yet.
Shimadzu and Agilent make very different systems. Perhaps after you decide on what type of system you need, you will be able to demo a similar system (hardware and software) to see if you have a preference. A true analytical system is very different from most semi-prep systems, but many analytical systems can be re-plumbed for semi-prep work with little effort. Establish your actual need and goals before you spend (waste) money.
Dear Phurpa,
I would suggest that you cast your net wide and consider many factors, such as sensitivity, drift, reliability, ease of use, ease of servicing and maintenance, manufacturers’ support, robustness, scalability etc.
Please read this feature.
http://go.labmanager.com/hplc-resource-guide
Kind regards,
Ade Kujore
Marketing
Cecil Instruments Limited
Milton Technical Centre, Cambridge CB24 6AZ
United Kingdom
email:- [email protected]
telephone:- +44 (0) 1223 420821
fax.:- +44 (0) 1223 420475
web site:- www.cecilinstruments.com
Hated Shimadzu software in 2002. Agilent can be customized to do anything you like nowadays for calculations and reporting. Lots of users worldwide, and extremely durable engineering. Our 1260 Infinity system has been analytical workhorse for vitamins and caffeine and special projects over 2 yr. Only problem was needed pump seals replaced (easy!)
Thank you all. The HPLC will be used mainly for preparative works - isolating compounds from crude mixture of plants and worms. Just wanted to know the efficiency, sensitivity and the durability. Both comes with the option to have UV-VIS or DAD (bit pricy).
@Phurpa-
DAD = diode array detection, with detection in the UV-vis range. Unless they are charging for the ability to see a spectrum, there really shouldn't be a price difference.
The ACCQPrep I mentioned earlier is specific for preparative work. As mentioned earlier, the Agilent can be customized but the large number of options, especially for analytical work, makes the user interface more difficult (I have a 1290 in my lab).
The attached poster describes how I can use an analytical run to generate a gradient for a prep run with natural products.
I lied / conveniently forgot, the Agilent 1260 Infinity, in 2 yr, also suffered a stuck check valve. However, sonication in MeOH freed it up just fine and works perfect. I learned dont leave line over weekend in buffer, but flush on Friday. How are you deciding how to cut your fractions? Bioassay, tlc, hplc? Having the diode array to see the spectrum is useful but if you already have a spectrophotometer and are purifying your candidates anyway, then seems the diode array is not crucial b/c you will get the spectrum eventually?
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