Hello, see attached paper may be helpful for you.

Good luck!

Hello, see attached paper may be helpful for you.

Good luck!

Please look at the following below links which may help you in your analysis:

http://www.latamjpharm.org/trabajos/28/3/LAJOP_28_3_1_10_662Q8GBZMX.pdf

http://www.globalresearchonline.net/journalcontents/volume11issue2/Article-004.pdf

Thanks

http://www.latamjpharm.org/trabajos/28/3/LAJOP_28_3_1_10_662Q8GBZMX.pdf

Dr, Isam Eldin Hussein Elgailani

I use Me:H2O mobile phase but the color of Schiff base diapers as indicator of hydrolysis of it; is it true? so a buffered solution may useful ?

The best is MeOH:Water 95:5,....but take care because the hydrolysis is real.....PBS can help you at used....

Following above all

Please have a look at these useful links.

https://www.pharmaguideline.com/2017/12/hplc-method-validation.html

Article HPLC METHOD VALIDATION FOR PHARMACEUTICALS: A REVIEW