HPLC columns mantenance ?

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¿Cómo evaluar eficazmente las intervenciones contra el maltrato infantil y optimizar el seguimiento de casos?

Con esta cuestión se busca hacer entender cuáles son los métodos y técnicas más eficaces para evaluar las intervenciones dirigidas a prevenir y tratar el maltrato infantil, así como mejorar el...

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¿Cómo diseñar estrategias de investigación participativa con menores en riesgo, respetando sus derechos?

Con esta cuestión se pretende crear conciencia sobre la importancia de utilizar estrategias con un enfoque centrado en los derechos de los participantes, en este caso, menores en riesgo de...

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Does anyone has experience preparing samples of lactose free yogurt to quantify sugars by HPLC-IR ?

I need some guidelines to prepare samples to analize and quantify sugars in free lactose yogurt using HPLC with refractive index detector.

14 February 2024 7,087 0 View

Do I need to make a heat shock for my PCr-tubes to ensure DNA unwanding ?

I did three PCR for the same DNA sample. The first and second one gave me a clear band band. However, in the last PCR I put the tubes in the PCR machine before it starts the heating cycle (25 °C)....

11 October 2023 4,139 0 View

How can I tell if two liquid compounds form a eutectic solvent?

For example, If I have lactic acid and glycerol how can I know the visual form?

21 September 2023 902 2 View

What causes cDNA degradation ?

After I make cDNA, I run an gel electrophoresis, but its band appears degraded. What can be the issue?

21 September 2023 4,212 1 View

Do you have issues running the light curve with your phyto US unit for Phyto PAM?

Hello you all, hope you are fine Well, I'm working right now meassuring different parameters of photosyntesis and I was interested in use a Phyto US unit for a PhytoPAM, the unit seems right and...

14 September 2023 788 0 View

Does Nuclease-Free Water act as Rnase inhibitor ?

I am little bit confuse between the composition and function of Nuclease-Free Water and Rnase-Inhibitor in RNA extraction .Does Nuclease-Free Water act as Rnase inhibitor ?

14 September 2023 9,472 3 View

Does anyone use the software CUE for workflows....any suggestions???

19 July 2023 5,719 1 View

Same gene sequence generate different leaves at phylogenetic tree, Why this happen?

i have made a phylogenetic tree to show the relationships betwen the ITS sequences from different Enterocytozoon bieneusi genotypes. I also have inputed a sequence obtained by our laboratory...

05 June 2023 8,777 10 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Bruce Neagle

The wash procedure for the column should be whatever the manufacturer recommended. Remember, it not just PHA's that are injected on the column but other crap from your sample matrix.

Lisbeth Mena

Does someone have experience with the Software CUE (for spectrophotometer) to develop a workflow.??? Any suggestion.??