Where can I find a dataset with convective heat transfer coefficients to train an IA?

Is there any dataset with heat transfer coefficients to train a neural network?

13 February 2019 3,235 2 View

In ABAQUS, How to define the lateral cyclic loading on the RC Column ?

I'm working in model consist of RC column subjected to lateral cyclic load in addition to constant axial load. in experimental paper rate of loading in lateral cyclic is 0.3 mm/second. and cyclic...

25 December 2018 4,632 3 View

How to measure the Specific Capacitance of a Supercapacitor using CV?

I have designed a paper-based electrochemical cell for my research. I use carbon nanotubes as working and counter electrodes, and I use Ag/AgCl as a reference electrode. I run and record cyclic...

22 July 2018 8,177 3 View

How to identify the source of sporadic/random contamination in my cell culture flasks?

I grow LNCaP prostate cancer cells in T flasks (vented neck, filter cap) in RPMI 1640 medium supplemented with 10% FBS and 1% Pen-Strep. While subculturing the cells, I am observing a strange...

04 March 2018 3,517 1 View

How could I extract DNA from brain tissue of rats?, more specifically from frontal cortex and striatum

thickness of the slides and which protocol to follow

03 August 2017 7,216 1 View

Which is an acceptable threshold of Soil Organic Matter (e.g., 1%; 1.5%; 2% of SOM) to sustain crop production in the Mediterranean ?

We are analyzing the soil organic matter (SOM) in cultivated soils in the Provence area under Mediterranean climate (500-800 mm/year) with my students in agroecology. Soils in the area are usually...

01 January 1970 7,039 12 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View