I need some guidelines to prepare samples to analize and quantify sugars in free lactose yogurt using HPLC with refractive index detector.
Con esta cuestión se busca hacer entender cuáles son los métodos y técnicas más eficaces para evaluar las intervenciones dirigidas a prevenir y tratar el maltrato infantil, así como mejorar el...
10 March 2024 2,828 1 View
Con esta cuestión se pretende crear conciencia sobre la importancia de utilizar estrategias con un enfoque centrado en los derechos de los participantes, en este caso, menores en riesgo de...
10 March 2024 2,427 0 View
I did three PCR for the same DNA sample. The first and second one gave me a clear band band. However, in the last PCR I put the tubes in the PCR machine before it starts the heating cycle (25 °C)....
11 October 2023 4,139 0 View
For example, If I have lactic acid and glycerol how can I know the visual form?
21 September 2023 902 2 View
After I make cDNA, I run an gel electrophoresis, but its band appears degraded. What can be the issue?
21 September 2023 4,212 1 View
Hello you all, hope you are fine Well, I'm working right now meassuring different parameters of photosyntesis and I was interested in use a Phyto US unit for a PhytoPAM, the unit seems right and...
14 September 2023 788 0 View
I am little bit confuse between the composition and function of Nuclease-Free Water and Rnase-Inhibitor in RNA extraction .Does Nuclease-Free Water act as Rnase inhibitor ?
14 September 2023 9,472 3 View
Does anyone use the software CUE for workflows....any suggestions???
19 July 2023 5,719 1 View
i have made a phylogenetic tree to show the relationships betwen the ITS sequences from different Enterocytozoon bieneusi genotypes. I also have inputed a sequence obtained by our laboratory...
05 June 2023 8,777 10 View
What its a recommendable procedure to develop a wash method in a phenyl column used to quantify PHAs by HPLC??
10 May 2023 7,009 2 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...
28 May 2024 9,382 4 View
I prepare M9 Agar plates with 0.4% Lactose for selection purposes, but the desired bacteria grow in a weird manner covering all the surface of the petri dish without being able to form distinct...
06 March 2024 1,099 1 View
We followed the NREL laboratory protocol to analyze reducing sugar after pretreatment of biomass via DNS method. But everytime we found the UV-Abs peak at 370 instead of 540nm. Suggestions are...
15 February 2024 2,605 0 View
Hello. I am currently trying to biotinylate some antibodies, and the antibody has a high amount of sucrose. Is there any documentation of inhibitory effects (or lack thereof) of sucrose (or other...
24 January 2024 6,490 0 View
For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars
27 November 2023 5,721 0 View
The reducing sugars were estimated by DNS method.
31 October 2023 9,915 0 View
The binding of the sugar-dye compound with a lectin should facilitate a shift in the emitted light that enables monitoring of lectin activity. Or perhaps there are other ways to monitor lectin...
29 October 2023 2,319 1 View
I would like to ask you about the method for sample storage, extraction, sample preparation and analysis of saccharides and sugar alcohols in needles and leaves of forest trees by LC-MS (or...
25 September 2023 3,064 4 View
I have seen numerous excellent research papers where metallic nanoparticles are used as carriers for enzymes or acids in lignocellulosic biomass pretreatment. But is it possible where the...
10 September 2023 1,445 4 View