How would you explain a real-time RT-PCR reaction with an efficiency of about 105%?

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No title

It is important for me to know the opinion from your point of view as researchers.

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Laurence Stuart Dawkins-Hall

hello Jorge

this can sometimes mean you are amplifying more than 1 specific product; invariably s second primer diner species: this will show as a second melt curve in your dissociation cycle at the end of your standard curve PCR cycle

Jorge Hernández-Bello

Thanks very much to all of you (Laurence, Jonci nad Anja), your answer was very heplful for me. Best regards to everyone.