Dear All,

I am wondering how the Transwell inserts can be recovered from fixing to be then embedded?

After fixing, I put each membrane on nitrocellulose membrane to avoid curling and then I leave them in an embedding cassette to be processed - automated protocol of our histology facility.

When embedding, I cut each membrane into half and put them upright. This process is very difficult because the Transwells start curling and they are very thin, so they don't settle very well. Also, when sectioning, Transwells would break because of their thickness.

Do you have any alternative on how to manage them before processing and then embed?

Thank you!

Aurora