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03 April 2019 4,304 6 View

Will washing and centrifugation disrupt the immune synapse?

I am working on a experiment to analyze the immune synapse between T cells and macrophages. In our protocol to stain the cells with fluorescent markers, we have quite a few pipetting, washing and...

08 September 2018 6,647 5 View

Should you use adherent or non-adherent RAW 264.7 mouse macrophage cells for phagocytosis experiments?

Hi! I am testing different vaccine adjuvants and their interaction with RAW 264.7 mouse macrophage cells. I am specifically interested in phagocytosis of the adjuvants. After phagocytosis, cell...

10 November 2017 3,692 3 View

What to pick for analyzing stimulated cell population: fold-change or background subtraction?

In our lab we have had discussions whether we should represent our flow cyotmetry data as fold-change or background subtracted. We vaccinate animals with antigens and later re-stimulate the...

06 July 2016 8,383 2 View

Are there journals that accept videos as publications?

Hi there! In our lab we shot some cool timelaps videos of parasitic worms under a high-resolution light microscope, showing how the eggs of these worms are moving trough their body and how they...

02 March 2016 9,899 0 View

What is the correct method to assign a value to samples that do not reach the baseline value in ELISA?

Hi guys, I am trying to find the best approach to assign a value to samples that do not reach the baseline value in ELISA. In my ELISA assay I perform a 2-fold serial dilution curve starting at a...

31 December 2015 3,056 9 View

Are non-tissue culture treated plates the ones to use when re-stimulating mouse splenocytes for Intracellular Cytokine Staining (ICS)?

I am looking into the plates that are optimal to re-stimulate murine splenocytes in, and subsequently use the same plate to perform ICS in. There are non-tissue culture treated plates...

07 August 2015 8,825 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View