For stable hemoglobin molecules it is necessary that alpha chain, beta chain and heme must be in balanced with ratio of 2:2:4 respectively. Imbalance between these ratios lead to instability in

hemoglobin quantity and quality. In beta thalassemia there is lack of beta chain synthesis while alpha chain is parallel synthesize. This free alpha chain in beta Thalassemia become precipitate with in red blood cells that lead to premature destruction of erythrocytes. Premature red cell destruction causes the ineffective erythropoiesis and hemolytic anemia. Quantity of free alpha chain in thalassemic patients is associated with clinical Consequences.